Polyphasic identification of decay agents of lemon fruits in Serbia

Lemon fruits are an important source of vitamin C, potassium, folate, carotenoids, polyphenols, coumarins and terpenes. These lemon compounds have antioxidant anti-inflammatory properties which beneficial effects on human health. This research aimed to elucidate the etiology blue green molds detected in Serbia. Using integrative identification approach, obtained isolates were characterized from morphological, physiological, molecular, phylogenetic pathological aspects. Colony growth morphology examined Czapek yeast autolysate agar (CYA), Malt extract (MEA) Creatine sucrose (CREA), CYA at two additional incubation temperatures (5 37 ?C). For molecular identification, ITS partial ?-tubulin (BenA) genes sequenced. Phylogenetic relationships investigated using maximum-likelihood method. A pathogenicity test was carried out possible difference among assessed with analysis variance (ANOVA) subsequent Tukey?s test. Four species identified: Penicillium expansum, digitatum, polonicum Talaromyces rugulosus. All four proved be pathogenic fruits, producing symptoms similar those observed naturally infected fruits. The results this study first records beforementioned Penicillium/ as postharvest pathogens Serbia world report T. rugulosus phytopathogenic same host.